WebMar 15, 2024 · Metabolic labeling experiments can be broadly divided into three classes: pulse-chase, approach-to-equilibrium, and other, more complicated strategies (Fig. 1). … WebMar 13, 2024 · Mice were injected intra-peritonealy with EdU at 0, 1, and 16 hours equivalent to a "pulse-chase-pulse" experiment. Thirty minutes after the last pulse, the mice were …

What Scientific Hypotheses Can Be Tested By A PulseChase …

A selected cell or a group of cells is first exposed to a labeled compound (the pulse) that is to be incorporated into a molecule or system that is studied (also see pulse labeling). The compound then goes through the metabolic pathways and is used in the synthesis of the product studied. For example, a radioactively labeled form of leucine ( H-leucine) can be supplied to a group of pancreatic beta cells, which then uses this amino acid in insulin synthesis. WebPulse/Chase Experimental Protocol. PLC 8/10/99. NOTE: This protocol is specific for labeling nascent tailspike polypeptide chains (and, as a control, Salmonella proteins) … indiana department of revenue retail merchant

Degradation Parameters from Pulse-Chase Experiments - PLOS

WebA commonly used approach is the "pulse-chase" experiment. One nice thing about pulse-chase experiments is that the same basic strategy can be used in many different … WebNov 3, 2014 · In particular, the unit focuses on the precise timing of pulse-chase experiments to evaluate early/short-time events in protein maturation in both suspended … WebPulse-chase experiments use labeled compounds to follow the dynamics of cellular processes and pathways. Molecules in a cell are continually being synthesized and degraded at various rates. Changes in molecule localizations and expression levels over time, can … indiana department of revenue tax form