WebApr 28, 2010 · Pull-down assays probe interactions between a protein of interest that is expressed as fusion protein (e.g., (e.g., bait) and the potential interacting partners (prey). … Webn GST-tagged proteins are highly suitable for pull-down assays n New Glutathione HiCap resin with up to 50% increased binding Purification of His-tagged proteins His-tagged …
Pathogens Free Full-Text Hsp90 Interacts with the Bacterial ...
Web[Abstract] GST pull-down assay is an effective way to examine the direct binding of two proteins in vitro. This protocol is based on the GST pull-down system from GE … Web1 Introduction. The glutathione S-transferase (GST) pull-down assay is a relatively easy, straightforward method to identify potential protein kinase C (PKC)-binding partners. The method is also extensively used to confirm … melanie thomas scott
A General Protocol for GST Pull-down
WebGeneral schematic of a pull-down assay. A pull-down assay is a small-scale affinity purification technique similar to immunoprecipitation, except that the antibody is replaced by some other affinity system. In this case, the affinity system consists of a glutathione S-transferase (GST)–, polyHis- or streptavidin-tagged protein or binding ... WebGST pulldown implies that at least one of your protein is produced and purified as a recombinant form (generally from E.coli) which requires additional steps and expertise. Some human proteins can ... WebThe pull-down assay is an in vitro technique used to detect physical interactions between two or more proteins and an invaluable tool for confirming a predicted protein-protein interaction or identifying novel interacting partners. This method typically involves the use of affinity purification with various wash and elution steps. melanie thomas now united