Web• add 4µl DIG high prime labeling mix; mix briefly and tap spin • incubate overnight at 37°C. • stop reaction by adding 2µl 0.2M EDTA (pH 8) and heat inactivate at 65°C 10min • boil … http://ipresci.com/uploads/allimg/20241128/1-21112R22I6491.pdf

DIG-High Prime DNA Labeling and Detection Starter Kit I from …

WebMar 30, 2007 · The membrane bound the DNA markers was cut out and was hybridized with DIG-labeled linear pBR322 DNA probes (obtained by digestion with EcoRI) at 40°C for 8 h according to the instruction manual of DIG High Prime DNA Labeling and Detection Starter Kit II (Roche Applied Science, Mannheim, Germany). The remaining membrane was … WebDNA labeling DIG-labeled DNA probes are generated with DIG-High Prime according to the random primed labeling technique. DIG-High Prime is a specially developed reaction … how are worksets best used

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WebThe DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by … WebDIG-High Prime Mixes 12 month; -15 to -25°C. Avoid repeated freezing and thawing! Benefit Feature Accurate and fast The use of premixed DIG-High Prime minimizes the hands-on-time required to label DNA probes and increases yields and reproducibility. Sensitive Single-copy genes can be detected in total human DNA complex and plant … how are worksheets labelled as by default