WebNov 27, 2006 · Cystatin F can inhibit AEP, but with lower affinity as cystatin E/M and C [80]. The recently elucidated crystal structure of cystatin F revealed that two N-linked glycosylation sites of cystatin F may modulate its inhibitory properties, in particular its reduced affinity towards AEP as compared to other cystatins [77] . WebCystatin E/M is a member of family 2 of the cystatin superfamily (1‑6). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice with a null mutation in the cystatin E/M gene have defects in epidermal cornification and die between 5 and 12 days of age (7). Cystatin E/M ...

Low-level internalization of cystatin E/M affects legumain …

WebThe most potent endogenous inhibitor of legumain is cystatin E/M, which is a secreted protein synthesised with an export signal. Therefore, we investigated the cellular interplay between legumain and cystatin E/M. As a cell model, HEK293 cells were transfected with legumain cDNA, cystatin E/M cDNA, or both, and over-expressing monoclonal cell ... WebApr 28, 2006 · Cystatin E/M expression strongly reduced tumor growth during the first 6–7 weeks after inoculation but had only a minor effect on incidence of metastasis and number of lesions in the lungs. Further, studies using the experimental lung colonization assay showed that cystatin E/M expression had no effect on the seeding and survival of tumor ... small tool zipper bag

Cystatins C, E/M and F in Human Pleural Fluids of Patients with ...

WebSep 9, 2016 · Introduction. Cystatin C (Cys C) , originally called as gamma-trace and post-gamma-globulin, is a non-glycosylated low molecular weight protein found in many body fluids and secretions. In 1961, it was isolated as a cerebrospinal fluid-specific protein in human and named as gamma-trace protein (γ-CSF) (Clausen 1961 ). Webcystatin E/M are type 2 cystatins and, together with cystatin F are the only cystatins capable of legumain inhibition (8). Typical type 2 cystatins are small molecules with a size of 13–14 kDa. They consist of 120-amino acid-residue-long polypeptides, which generally show a high degree of sequence WebCystatin E/M is a member of family 2 of the cystatin superfamily (1‑6). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as … small tool storage sheds