Cryaa promoter
WebJun 5, 2015 · CRYAA transcript and protein levels are both downregulated, and the CpG island in the CRYAA promoter is hypermethylated in human age-related cataract (Zhou et al., Reference Zhou, Luo, Liu, Fan and Lu 2012). We performed promoter methylation analysis of six genes, including TRAM1, CRYAA, HSF4, VEGFA, GJA3, and FTL. … WebMar 17, 2016 · A 1111 bp region of the human CRYAA proximal promoter was amplified using CRYAA-Promoter-F and CRYAA-Promoter-R as described above. The PCR product was cloned into the PCR 2.1-TOPO (Invitrogen ...
Cryaa promoter
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WebDec 3, 2013 · DNA methylation was analyzed by pyrosequencing the CpG islands in CRYAA promoter. CRYAA expression was compared among high-myopic eyes with … WebDec 1, 2024 · Notably, the adenovirus E1b promoter, the zebrafish lepb 0.8-kb (P0.8) and lepb 2-kb (P2) promoters, and a new zebrafish synthetic promoter (ZSP) that combines elements of the E1b and P0.8 promoters drove little or no ectopic expression, making them suitable for transgenic assays. We also found significant differences in compatibility …
WebDec 2, 2016 · Whereas in the mouse the γ-crystallin mutants were under the control of their respective native promoters, the use of the αA-crystallin (cryaa) promoter to drive the expression in the zebrafish lens may reduce the overall concentration of these proteins. This may allow the distinction of intrinsic differences in the aggregation propensity of ... WebSV40 polyA, zebrafish cryaa promoter (Kurita et al., 2003), AmCyan, SV40 polyA. For the myl7:Cre, cryaa:YPetulm13Tg line (abbreviated myl7:Cre), the following elements were assembled by PCR-based cloning techniques in a vector containing MiniTol2 repeats for transgenesis: 5.1 kb fragment of the zebrafish myl7 promoter, Cre recombinase
WebDec 1, 2024 · Promoters of ubiquitously expressed genes exhibit strong intrinsic activity and low responsiveness to enhancers, while promoters of genes that are variably … Web035233 STOCK Tg(Cryaa-cre)10Mlr/J MLR10 transgenic mice exhibit cre recombinase expression in both the lens epithelium and lens fibers under the transcriptional control of the mouse Cryaa (crystallin, alpha A) promoter modified by the insertion of a Pax6 consensus binding element/lens epithelial cell enhancer. Cre expression is not found in other parts …
WebPromoter SP6 and CMV Availability Industry, Academic Institutions, and Nonprofits pE-SUMO CRYAA Plasmid #80756 Purpose Expression of human alpha A-crystallin in E. …
WebThe study demonstrates that residues 70-88 in CRYAA act as a primary substrate binding site and account for the bulk of the total chaperone activity. The T allele of rs7278468 in the crystallin alpha A promoter is associated with age-related cataract through increasing binding of KLF-10 and thus decreasing crystallin alpha A transcription. garnett chamber of commerce garnett ksWebFigure Legend Snippet: A. Schematic representation of elements in the Tol2-based transposon vector driving the expression of oncogenic GNAQ Q209P under the control of … black sabbath i was born without yaWebMar 17, 2016 · The CRYAA promoter has transcriptional activity in HLE cells: compared with the vector, the luciferase activity was more than 10 times higher in cells transfected … black sabbath i\u0027m going through changesWebDec 3, 2013 · CpG islands in lens epithelial CRYAA promoter in the HMC NC5-6 Group exhibited the highest methylation of all the groups, but no statistically significant differences were evident between the HMC ... black sabbath junior\\u0027s eyesWebPolymorphisms in alpha A crystallin (CRYAA) gene have been implicated in susceptibility to cataracts, but some published studies have reported inconclusive results. Our study … black sabbath john peelWebThe mRNA and protein levels of CRYAA were significantly reduced in the lens epithelia of age-related nuclear cataract cases vs. age-matched controls, which corresponded to hypermethylation of the CpG island of CRYAA promoter. Treatment with a DNA-demethylating agent was associated with restoration of CRYAA expression. garnett close watfordWebAug 9, 2016 · CpG methylation of CRYAA promoter decreases the DNA-binding capacity of transcription factor SP-1. EMSA experiments show the specific binding of Sp1 to the binding sequence of the CRYAA promoter. Lane 1: biotin-labeled wild-type Sp1 probe incubated with HLE B-3 nuclear extracts. Sp1 binding is depicted by arrows on the left of the figures. black sabbath i won\\u0027t cry for you lyrics